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Transient transfection using ambr
and Life Technologies' Expi293™

ambr - system overview
ambr™ mimics the characteristics of classical bioreactors at microscale (10-15ml), by using cost effective, disposable micro bioreactors, controlled by an automated workstation.
  ambr enables the rapid evaluation of multiple bioreactor cultures at microscale, increasing productivity in cell line development with significant savings on materials and labour.

Central to the system is the new ambr micro bioreactor – specifically designed to mimic the characteristics of a larger scale bioreactor.
  To learn more about the unique ambr micro bioreactor design, and to view key processes from load of media to sampling - take an interactive tour.
  ambr micro bioreactor 'Interactive Tour'


The ambr workstation controls 24 or 48 disposable bioreactors, offering parallel processing and evaluation of multiple experiments in an automated benchtop system.
  • Closed loop control of pH and DO with independent control of O2 and CO2 for each vessel
  • Automated liquid handling for reactor set-up, feeds, base addition and sampling
  • Easy installation into laminar flow hood allowing aseptic operation
  • Optional integrated Vi-CELL® cell viability analysis
  • A better tool for cell line selection and development - closely mimics the conditions of larger scale bioreactors
  • Simplified adoption of Quality by Design (QbD) principles for cell culture development
  • Significant reduction in cost per experiment
  • More experiments with less operator time for set up and clean down
  • Cell line selection and stability
  • Media feed strategies
  • Transient transfection
  • Process robustness experiments supporting ‘quailty by design‘ studies
  • Automation of DOE approaches to process optimisation
  ambr offers an accurate and cost-effective approach to replicating bioreactor conditions, and can be used as a microscale model for a wide range of upstream processes such as cell line selection and process optimisation.
  For more information on ambr:
  Get more detail on the system's key applications:
process development & scale up and cell line selection & development
  To discuss your requirements please contact the TAP team
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