RAFT
(Real Architecture for 3D Tissue)
is a new system to enable scientists to
create complex 3D cell cultures from collagen,
in a convenient, simple-to-use format.
Developed by
TAP in collaboration with leading tissue
engineering academics, RAFT
combines a novel patented process
with a range of sterile consumables to create
realistic 3D cell cultures in a simple,
consistent and reproducible format.
Using
the
RAFT system, it
takes
less than one hour
to create up to 96 cell
cultures simultaneously.
To learn
more, view the
RAFT process animation
which provides
a simulation of the process
- from culture preparation,
set up and incubation,
through to the creation
of the final culture.
Simple to use
system with standard labware and protocols
that enable the production of robust, reproducible
3D cultures in a 96-well plate format.
Novel RAFT
Plate – a custom microplate
with biocompatible absorbers to control
the concentration of cells and collagen
to generate the cultures
Choice of
96-well culture plates –
standard clear plates for a broad range
of applications and black walled/clear
bottomed plates for fluorescent or luminescent
readouts
RAFT Reagent
kit - containing sufficient quantities
of reagents (Type I collagen, neutralising
solution and 10x MEM solution) to create
up to four plates of 96 cell cultures
using our standard supplied protocol
Specially designed
plate heater – Suitable
for use in a laminar flow hood, with
a small footprint and smooth, easily
cleaned or sprayed surfaces
The
RAFT plate contains the absorbers
that control the concentration
of the cells and collagen
The
RAFT plate in use with black
walled/clear bottomed plates
for fluorescent or luminescent
readouts
Creates a more realistic natural
environment for cells and allows scientists
to study their complex behaviour
Enables production of consistent,
reproducible 3D cell cultures using
a simple process and equipment
Allows scientists to take complete
control of their experiment - using
their own cell type(s), selecting the
seeding density and enabling complex
3D cell culture in a controlled manner
Suitable for use with a broad range
of analytical techniques such as imaging,
biochemical, histological and ‘omics
techniques
RAFT
can be used as a platform technology to
support assay development and secondary
screening programs, as well as a broad range
of cell biology research areas.
