| |
The following
has been kindly supplied by the Centre National
de Genotypage and formed part of an application
note produced in conjunction with TAP. It provides
an illustration of how BasePlate is being used within
the genotyping environment. |
| |
|
| |
PCR is a central tool
for high throughput genomics analysis. As the throughput
requirements in areas such as genotyping, expression
studies, association studies and sequencing increase,
the set-up and performance of large numbers of PCRs
at low volumes becomes a bottleneck in these processes. |
| |
The photograph below
illustrates the products from a high speed PCR set-up
in a 5ul final volume in a384-well plates.
BasePlate’s flexibility can be extended to
produce PCR reactions in 32x384 well plates, allowing
for the set-up of 12,000 reactions in under 1 hour. |
| |
The PCRs(1)
were set-up with alternate wells containing template
DNA. The resulting PCR products were run out on
3% agarose gels: |
| |
 |
| |
This demonstrates the
consistency and success of the PCR set-ups –
the BasePlate liquid handling system ensuring reliable
and reproducible results with no cross contamination.
|
| |
The PCRs were executed
on fragments in the human B-2-adrenergic
receptor gene, which belongs to a family of seven-transmembrane
receptors(2).
SNPs in this receptor are associated with a genetic
predisposition to hypertension and are found to
be relevant for cardiovascular disease(3) |
| |
References:
(1) The PCR products
are amplified on a GeneAmp PCR System 9700 Perkin
Thermocycler
(2) Johnson M
(1998) Am J Respir Crit Care Med, 158, 146-153
(3) Timmermann
B, Mo R, Luft FC, Gerdts E, Busjahn
|
| |
|
